For years I have written about Doxycycline. I have mentioned how my mentor at Harvard, Dr. Judah Folkman, used to comment that 20mg is the best for decreasing inflammation and that if he could put it in the water, he would. He used to note that a majority of his cancer patients were on 20mg Doxy to help decrease inflammation (and VEGF: see Reference below).
We have used Doxycycline 20mg po qday for years to help decrease eyelid inflammation. It does its job but not everyone notices an improvement in their eye symptoms. A great deal of Meibomian Gland atrophy is due to inflammation at the gland’s orifice which lead to a backlog of oil and ultimate downregulation of the oil production and atrophy.
One of the key questions we have long had is the effect of Doxycyline on gut flora and resistance. A wonderful patient did the research for me and found this article noting 20mg of Doxy does not affect the microbiome which is a key component to general health (see next blog post 😉
Doxycycline modulates VEGF-A expression: Failure of doxycycline-inducible lentivirus shRNA vector to knockdown VEGF-A expression in transgenic mice
Anindita Das, Editor
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Vascular endothelial growth factor-A (VEGF-A) is the master regulator of angiogenesis, vascular permeability and growth. However, its role in mature blood vessels is still not well understood. To better understand the role of VEGF-A in the adult vasculature, we generated a VEGF-A knockdown mouse model carrying a doxycycline (dox)-regulatable short hairpin RNA (shRNA) transgene, which silences VEGF-A. The aim was to find the critical level of VEGF-A reduction for vascular well-being in vivo. In vitro, the dox-inducible lentiviral shRNA vector decreased VEGF-A expression efficiently and dose-dependently in mouse endothelial cells and cardiomyocytes. In the generated transgenic mice plasma VEGF-A levels decreased shortly after the dox treatment but returned back to normal after two weeks. VEGF-A expression decreased shortly after the dox treatment only in some tissues. Surprisingly, increasing the dox exposure time and dose led to elevated VEGF-A expression in some tissues of both wildtype and knockdown mice, suggesting that dox itself has an effect on VEGF-A expression. When the effect of dox on VEGF-A levels was further tested in naïve/non-transduced cells, the dox administration led to a decreased VEGF-A expression in endothelial cells but to an increased expression in cardiomyocytes. In conclusion, the VEGF-A knockdown was achieved in a dox-regulatable fashion with a VEGF-A shRNA vector in vitro, but not in the knockdown mouse model in vivo
Low dose Doxycycline 20mg per day: Periostat: Is extremely safe. Rare side effect which I have never seen with doxycycline but has been reported at higher levels.
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