van Luijk P, Pringle S, Deasy JO, Moiseenko VV, Faber H, Hovan A, Baanstra M, van der Laan HP, Kierkels RG, van der Schaaf A, Witjes MJ, Schippers JM, Brandenburg S, Langendijk JA, Wu J, Coppes RP.

Sci Transl Med. 2015 Sep 16;7(305):305ra147. doi: 10.1126/scitranslmed.aac4441.

PMID:: 26378247

Free PMC Article

Similar articles

Select item 2599519126.

Brooke-Spiegler syndrome presenting multiple concurrent cutaneous and parotid glandneoplasms: cytologic findings on fine-needle sample and description of a novel mutation of the CYLD gene.

Malzone MG, Campanile AC, Losito NS, Longo F, Perri F, Caponigro F, Schiavone C, Ionna F, Maiello F, Martinuzzi C, Nasti S, Botti G, Fulciniti F.

Diagn Cytopathol. 2015 Aug;43(8):654-8. doi: 10.1002/dc.23275. Epub 2015 May 21.

PMID:: 25995191

Similar articles

Select item 2562562327.

Silk fibroin scaffolds promote formation of the ex vivo niche for salivary gland epithelial cell growth, matrix formation, and retention of differentiated function.

Zhang BX, Zhang ZL, Lin AL, Wang H, Pilia M, Ong JL, Dean DD, Chen XD, Yeh CK.

Tissue Eng Part A. 2015 May;21(9-10):1611-20. doi: 10.1089/ten.TEA.2014.0411. Epub 2015 Mar 9.

PMID:: 25625623

Free PMC Article

Similar articles

Select item 2499335428.

Improvement in the repair of defects in maxillofacial soft tissue in irradiated minipigs by a mixture of adipose-derived stem cells and platelet-rich fibrin.

Chen Y, Niu Z, Xue Y, Yuan F, Fu Y, Bai N.

Br J Oral Maxillofac Surg. 2014 Oct;52(8):740-5. doi: 10.1016/j.bjoms.2014.06.006. Epub 2014 Jun 30.

PMID:: 24993354

2017 Aug 16;10:295-299. doi: 10.2147/IMCRJ.S142514. eCollection 2017.

First-in-man intraglandular implantation of stromal vascular fraction and adipose-derived stem cells plus platelet-rich plasma in irradiation-induced gland damage: a case study.

Comella K1, Bell W2.

Author information

Abstract

BACKGROUND:

Stromal vascular fraction (SVF) is a mixture of cells which can be isolated from a mini-lipoaspirate of fat tissue. Platelet-rich plasma (PRP) is a mixture of growth factors and other nutrients which can be obtained from peripheral blood. Adipose-derived stem/stromalcells (ADSCs) can be isolated from fat tissue and expanded in culture. The SVF includes a variety of different cells such as ADSCs, pericytes, endothelial/progenitor cells, and a mix of different growth factors. The adipocytes (fat cells) can be removed via centrifugation. Here, we describe the rationale and, to our knowledge, the first clinical implementation of SVF and PRP followed by repeat dosing of culture-expanded ADSCs into a patient with severe xerostomia postirradiation.

METHODS:

Approximately 120 mLs of adipose tissue was removed via mini-lipoaspirate procedure under local anesthetic. The SVF was prepared from half of the fat and resuspended in PRP. The mixture was delivered via ultrasound directly into the submandibular and parotidglands on both the right and left sides. The remaining 60 mLs of fat was processed to culture-expand ADSCs. The patient received seven follow-up injections of the ADSCs plus PRP at 5, 8, 16, 18, 23, 28, and 31 months postliposuction. The subject was monitored over a period of 31 months for safety (adverse events), glandular size via ultrasound and saliva production.

RESULTS:

Throughout the 31-month monitoring period, no safety events such as infection or severe adverse events were reported. The patient demonstrated an increase in gland size as measured by ultrasound which corresponded to increased saliva production.

CONCLUSION:

Overall, the patient reported improved quality of life and willingness to continue treatments. The strong safety profile and preliminary efficacy results warrant larger studies to determine if this is a feasible treatment plan for patients postradiation.

2009 Sep;92(3):466-71. doi: 10.1016/j.radonc.2009.06.023. Epub 2009 Jul 20.

Isolation and characterization of human salivary gland cells for stem cell transplantation to reduce radiation-induced hyposalivation.

Feng J1, van der Zwaag M, Stokman MA, van Os R, Coppes RP.

Author information

Abstract

BACKGROUND:

Recently, we showed that transplantation of 100-300 c-Kit(+) stem cells isolated from cultured salispheres ameliorates radiation-damage in murine salivary glands. The aim of this study is to optimize and translate these findings from mice to man.

METHODS:

Mouse and human non-malignant parotid and submandibular salivary gland tissue was collected and enzymatically digested. The remaining cell suspension was cultured according to our salisphere culture method optimized for murine salispheres. Salisphere cells were tested using 3D matrix culturing for their in vitro stem cell characteristics such as the potential to differentiate into tissue specific cell types. Several potential mouse and human salivary gland stem cells were selected using FACS.

RESULTS:

In human salivary gland, c-Kit(+) cells were only detected in excretory ducts as shown previously in mice. From both human parotid and submandibular gland cell suspensions salispheres could be grown, which when placed in 3D culture developed ductal structures and mucin-expressing acinar-like cells. Moreover, cells dispersed from primary salispheres were able to form secondary spheres in matrigel, a procedure that could be repeated for at least seven passages. Approximately 3000 c-Kit+ cells could be isolated from primary human salispheres per biopsy.

CONCLUSION:

Human salivary glands contain a similar ‘putative’ stem cell population as rodents, expressing c-kit and capable of in vitro differentiation and self-renewal. In the future, these cells may have the potential to reduce radiotherapy-induced salivary gland dysfunction in patients.