Culturing Conjunctival Cells from a Patient’s Own Stem Cells: the Next Frontier in Pterygium Surgery
Our next goal is to grow a patient’s conjunctival cells from his or her own stem cells.
Eventually I would like to grow stem cells to see if we can regrow Meibomian Glands which have proven to be vital in one not feeling one’s eyes daily.
Sandra Lora Cremers, MD, FACS
References:
Human Conjunctival Stem Cells are Predominantly Located in the Medial Canthal and Inferior Forniceal Areas.
1 Department of Eye and Vision Science, University of Liverpool, Liverpool, United Kingdom St. Paul’s Eye Unit, Royal Liverpool University Hospital, Liverpool, United Kingdom.2 Department of Eye and Vision Science, University of Liverpool, Liverpool, United Kingdom.3 Department of Eye and Vision Science, University of Liverpool, Liverpool, United Kingdom Department of Biostatistics, University of Liverpool, Liverpool, United Kingdom.
Abstract
PURPOSE:
The conjunctiva plays a key role in ocular surface defence and maintenance of the tear film. Ex vivo expansion of conjunctival epithelial cells offers potential to reconstruct the ocular surface in cases of severe cicatrising disease, but requires initial biopsies rich in stem cells to ensure long-term success. The distribution of human conjunctival stem cells, however, has not been clearly elucidated.
METHODS:
Whole human cadaveric conjunctiva was retrieved and divided into specific areas for comparison. From each donor, all areas from one specimen were cultured for colony-forming efficiency assays and immunocytochemical studies; all areas from the other specimen were fixed and paraffin embedded for immunohistochemical studies. Expression of CK19, p63, and stem cell markers ABCG2, ΔNp63, and Hsp70 were analyzed. Results were correlated to donor age and postmortem retrieval time.
RESULTS:
Conjunctiva was retrieved from 13 donors (26 specimens). Colony-forming efficiency and expression of stem cell markers ABCG2, ΔNp63, and Hsp70 in cultures and ABCG2 in fixed tissue were all consistently demonstrated throughout the tissue but with highest levels in the medial canthal and inferior forniceal areas (P < 0.01 for each). Both increasing donor age and longer postmortem retrieval times were associated with significantly lower colony-forming efficiency, stem cell marker expression in cell cultures and ABCG2 expression in fixed tissue.
CONCLUSIONS:
Biopsies from the medial canthus and inferior forniceal areas, from younger donors, and with short postmortem retrieval times offer the greatest potential to developing conjunctival stem cell-rich epithelial constructs for transplantation.
Copyright 2015 The Association for Research in Vision and Ophthalmology, Inc.
KEYWORDS:
conjunctiva; inferior fornix; medial canthus; progenitor cell; stem cell
1.
Spaniol K, Holtmann C, Geerling G, Schrader S.
Ophthalmologe . 2016 Dec 22. doi: 10.1007/s00347-016-0419-1. [Epub ahead of print] German.
Select item 27693410 2.
Yu M, Bojic S, Figueiredo GS, Rooney P, de Havilland J, Dickinson A, Figueiredo FC, Lako M.
Exp Eye Res . 2016 Nov;152:113-122. doi: 10.1016/j.exer.2016.09.008.
Select item 27665215 3.
Busin M, Breda C, Bertolin M, Bovone C, Ponzin D, Ferrari S, Barbaro V, Elbadawy HM.
Ophthalmology . 2016 Dec;123(12):2481-2488. doi: 10.1016/j.ophtha.2016.08.018.
Select item 27612554 4.
Ali M, Shah D, Pasha Z, Jassim SH, Jaboori AJ, Setabutr P, Aakalu VK.
Curr Eye Res . 2016 Sep 9:1-7. [Epub ahead of print]
Select item 27367746 5.
Yeung AM, Faraj LA, McIntosh OD, Dhillon VK, Dua HS.
Eye (Lond) . 2016 Oct;30(10):1389-1394. doi: 10.1038/eye.2016.127.
Select item 27215000 6.
Baiula M, Spampinato S.
Eye Sci . 2015 Dec;30(4):140-2. No abstract available.
Select item 26971683 7.
Dhamodaran K, Subramani M, Matalia H, Jayadev C, Shetty R, Das D.
Cytotherapy . 2016 Apr;18(4):546-61. doi: 10.1016/j.jcyt.2016.01.003.
Select item 26938569 8.
Utheim TP, Utheim ØA, Khan QE, Sehic A.
J Funct Biomater . 2016 Mar 1;7(1). pii: E5. doi: 10.3390/jfb7010005. Review.
Select item 26555593 9.
Kang BS, Kim MK, Wee WR, Oh JY.
Cornea . 2016 Jan;35(1):51-5. doi: 10.1097/ICO.0000000000000677.
Select item 26451366 10.
Dobrowolski D, Orzechowska-Wylegala B, Wowra B, Wroblewska-Czajka E, Grolik M, Szczubialka K, Nowakowska M, Puzzolo D, Wylegala EA, Micali A, Aragona P.
Biomed Res Int . 2015;2015:281870. doi: 10.1155/2015/281870.
Select item 26344231 11.
Nadri S, Yazdani S.
Curr Protoc Stem Cell Biol . 2015 May 1;33:1F.14.1-8. doi: 10.1002/9780470151808.sc01f14s33.
Select item 26343740 12.
Sehic A, Utheim ØA, Ommundsen K, Utheim TP.
J Funct Biomater . 2015 Aug 28;6(3):863-88. doi: 10.3390/jfb6030863. Review.
Select item 26283864 13.
Dhamodaran K, Subramani M, Jeyabalan N, Ponnalagu M, Chevour P, Shetty R, Matalia H, Shetty R, Prince SE, Das D.
Mol Vis . 2015 Jul 31;21:828-45.
Select item 26203759 14.
Holland EJ.
Cornea . 2015 Oct;34 Suppl 10:S9-15. doi: 10.1097/ICO.0000000000000534. Review.
Select item 26193922 15.
Thomasen H, Müller B, Poetsch M, Steuhl KP, Meller D.
Invest Ophthalmol Vis Sci . 2015 Jul;56(8):4460-9. doi: 10.1167/iovs.14-15888.
Select item 26142953 16.
Dziasko MA, Tuft SJ, Daniels JT.
Exp Eye Res . 2015 Sep;138:70-9. doi: 10.1016/j.exer.2015.06.026.
Select item 25889475 17.
Bobba S, Chow S, Watson S, Di Girolamo N.
Stem Cell Res Ther
Select item 25652765 18.
Su W, Wan Q, Huang J, Han L, Chen X, Chen G, Olsen N, Zheng SG, Liang D.
J Allergy Clin Immunol . 2015 Aug;136(2):423-32.e8. doi: 10.1016/j.jaci.2014.12.1926.
Select item 25384970 19.
Silber PC, Ricardo JR, Cristovam PC, Hazarbassanov RM, Dreyfuss JL, Gomes JA.
Eur J Ophthalmol . 2014 Nov 6:0. doi: 10.5301/ejo.5000511. [Epub ahead of print]
Select item 25380529 20.
Massie I, Dale SB, Daniels JT.
Tissue Eng Part C Methods . 2015 Jun;21(6):576-84. doi: 10.1089/ten.TEC.2014.0458.
Select item 25158157 21.
Li DQ, Wang Z, Yoon KC, Bian F.
J Stem Cells . 2014;9(2):79-91. Review.
Select item 24769908 22.
Sugiyama H, Yamato M, Nishida K, Okano T.
Mol Ther . 2014 Aug;22(8):1544-55. doi: 10.1038/mt.2014.69.
Select item 24750037 23.
Eidet JR, Utheim ØA, Islam R, Lyberg T, Messelt EB, Dartt DA, Utheim TP.
Curr Eye Res . 2015 Jan;40(1):30-9. doi: 10.3109/02713683.2014.909497.
Select item 24492934 24.
Yang J, Li Y, Erol D, Wu WH, Tsai YT, Li XR, Davis RJ, Tsang SH.
Graefes Arch Clin Exp Ophthalmol . 2014 Mar;252(3):423-31. doi: 10.1007/s00417-014-2575-9.
Select item 24382239 25.
Chen GL, Xiao Y, Du YY, Liu YL, Liu ZY, Zhang H.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao . 2013 Dec;35(6):623-7. doi: 10.3881/j.issn.1000-503X.2013.06.007. Chinese.
Select item 24357922 26.
Jeon S, Choi SH, Wolosin JM, Chung SH, Joo CK.
Mol Vis . 2013 Dec 16;19:2542-50.
Select item 24269413 27.
Rivas L, Blázquez A, Muñoz-Negrete FJ, López S, Rebolleda G, Domínguez F, Pérez-Esteban A.
Arch Soc Esp Oftalmol . 2014 Jan;89(1):10-6. doi: 10.1016/j.oftal.2013.07.013. Spanish.
Select item 23981725 28.
Miyashita H, Yokoo S, Yoshida S, Kawakita T, Yamagami S, Tsubota K, Shimmura S.
Stem Cells Transl Med
Select item 23588957 29.
Nadri S, Kazemi B, Eslaminejad MB, Yazdani S, Soleimani M.
Mol Biol Rep . 2013 Jun;40(6):3883-90. doi: 10.1007/s11033-012-2360-y. Erratum in: Mol Biol Rep. 2013 Jul;40(7):4673. Eeslaminejad, Mohamadreza Baghaban [corrected to Eslaminejad, Mohamadreza Baghaban].
Select item 23559315 30.
Sejpal K, Ali MH, Maddileti S, Basu S, Ramappa M, Kekunnaya R, Vemuganti GK, Sangwan VS.
JAMA Ophthalmol . 2013 Jun;131(6):731-6. doi: 10.1001/jamaophthalmol.2013.2308.
Select item 23129317 31.
Osei-Bempong C, Figueiredo FC, Lako M.
Bioessays . 2013 Mar;35(3):211-9. doi: 10.1002/bies.201200086. Review.
Select item 22937499 32.
Xu B, Fan TJ, Zhao J, Sun A, Wang RX, Hu XZ, Yu HZ, Fan XY, Xu XH.
Int J Ophthalmol . 2012;5(4):424-9. doi: 10.3980/j.issn.2222-3959.2012.04.04.
Select item 22933392 33.
Schrader S, O’Callaghan AR, Tuft SJ, Beaconsfield M, Geerling G, Daniels JT.
J Tissue Eng Regen Med . 2014 Dec;8(12):969-77. doi: 10.1002/term.1599.
Select item 22914953 34.
Corradini F, Venturi B, Pellegrini G, De Luca M.
Methods Mol Biol . 2012;916:357-72. doi: 10.1007/978-1-61779-980-8_26.
Select item 22913741 35.
Schrader S, Tuft SJ, Beaconsfield M, Borrelli M, Geerling G, Daniels JT.
Curr Eye Res . 2012 Dec;37(12):1067-74. doi: 10.3109/02713683.2012.713155.
Select item 22784272 36.
Eslani M, Baradaran-Rafii A, Ahmad S.
Semin Ophthalmol . 2012 May-Jul;27(3-4):80-93. doi: 10.3109/08820538.2012.680641.
Select item 22265153 37.
Basu S, Ali H, Sangwan VS.
Am J Ophthalmol . 2012 Apr;153(4):643-50, 650.e1-2. doi: 10.1016/j.ajo.2011.09.016.
Select item 22050528 38.
Mason SL, Stewart RM, Kearns VR, Williams RL, Sheridan CM.
Regen Med . 2011 Nov;6(6):767-82. doi: 10.2217/rme.11.94. Review.
Select item 22027678 39.
Robles-Contreras A, Vizuet L, Rivera E, Serafin-López J, Estrada-Garcia I, Estrada-Parra S, Chávez R, Garfias Y, Perez-Tapia M, Jiménez-Martínez MC.
Rev Alerg Mex . 2011 May-Jun;58(3):147-54. Spanish.
Select item 21642849 40.
Daya SM, Chan CC, Holland EJ; Members of The Cornea Society Ocular Surface Procedures Nomenclature Committee..
Cornea . 2011 Oct;30(10):1115-9. doi: 10.1097/ICO.0b013e318207f135.
Select item 21418932 41.
Li WW, Sun XG.
Zhonghua Yan Ke Za Zhi . 2011 Jan;47(1):79-82. Review. Chinese.
Select item 21403854 42.
Amirjamshidi H, Milani BY, Sagha HM, Movahedan A, Shafiq MA, Lavker RM, Yue BY, Djalilian AR.
Mol Vis . 2011 Mar 8;17:658-66.
Select item 21354508 43.
Pascual G, Montes MA, Pérez-Rico C, Pérez-Kohler B, Bellón JM, Buján J.
Arch Soc Esp Oftalmol . 2010 Dec;85(12):400-4. doi: 10.1016/j.oftal.2010.11.016. Spanish.
Select item 21271034 44.
Ricardo JR, Gomes JA.
Arq Bras Oftalmol . 2010 Nov-Dec;73(6):541-7. Review. Portuguese.
Select item 22384653 45.
Dobrowolski D, Wylegala E, Orzechowska-Wylegala B, Wowra B, Wróblewska-Czajka E.
Klin Oczna . 2011;113(10-12):346-51.
Select item 21179428 46.
Li W, Sun X, Wang Z, Li R, Li L.
Mol Vis . 2010 Dec 15;16:2739-44.
Select item 21082888 47.
Schrader S, Notara M, Tuft SJ, Beaconsfield M, Geerling G, Daniels JT.
Regen Med . 2010 Nov;5(6):877-89. doi: 10.2217/rme.10.73.
Select item 20712971 48.
Di Iorio E, Ferrari S, Fasolo A, Böhm E, Ponzin D, Barbaro V.
Ocul Surf . 2010 Jul;8(3):146-53.
Select item 20573916 49.
Rama P, Matuska S, Paganoni G, Spinelli A, De Luca M, Pellegrini G.
N Engl J Med . 2010 Jul 8;363(2):147-55. doi: 10.1056/NEJMoa0905955.
Select item 20489583 50.
Colabelli Gisoldi RA, Pocobelli A, Villani CM, Amato D, Pellegrini G.
Cornea . 2010 Jul;29(7):715-22. doi: 10.1097/ICO.0b013e3181c91ac4.
Select item 20387535 51.
Kinoshita S.
Nippon Ganka Gakkai Zasshi . 2010 Mar;114(3):161-99; discussion 200-1. Review. Japanese.
Select item 19920075 52.
Cheng CC, Wang DY, Kao MH, Chen JK.
J Cell Sci . 2009 Dec 15;122(Pt 24):4473-80. doi: 10.1242/jcs.054791.
Select item 19761393 53.
Schrader S, Notara M, Beaconsfield M, Tuft S, Geerling G, Daniels JT.
Regen Med . 2009 Sep;4(5):677-87. doi: 10.2217/rme.09.39.
Select item 19466341 54.
Gomes JA, Pazos HS, Silva AB, Cristovam PC, Belfort Júnior R.
Arq Bras Oftalmol . 2009 Mar-Apr;72(2):254-6. Portuguese.
Select item 19324848 55.
Akinci MA, Turner H, Taveras M, Barash A, Wang Z, Reinach P, Wolosin JM.
Invest Ophthalmol Vis Sci . 2009 Sep;50(9):4162-72. doi: 10.1167/iovs.08-2861.
Select item 19251317 56.
Low SP, Voelcker NH, Canham LT, Williams KA.
Biomaterials . 2009 May;30(15):2873-80. doi: 10.1016/j.biomaterials.2009.02.008.
Select item 19003195 57.
O’Sullivan F, Clynes M.
Cytotechnology . 2007 Apr;53(1-3):101-6. doi: 10.1007/s10616-007-9063-6.
Select item 18851950 58.
Nadri S, Soleimani M, Mobarra Z, Amini S.
Biochem Biophys Res Commun . 2008 Dec 12;377(2):423-8. doi: 10.1016/j.bbrc.2008.09.148.
Select item 18602223 59.
Auxenfans C, Builles N, Andre V, Lequeux C, Fievet A, Rose S, Braye FM, Fradette J, Janin-Manificat H, Nataf S, Burillon C, Damour O.
Pathol Biol (Paris) . 2009 Jun;57(4):290-8. doi: 10.1016/j.patbio.2008.04.014. Review. French.
Select item 18334934 60.
Madhira SL, Vemuganti G, Bhaduri A, Gaddipati S, Sangwan VS, Ghanekar Y.
Mol Vis . 2008 Jan 30;14:189-96.
Select item 17939121 61.
Rosellini A, Papini S, Giannarini C, Nardi M, Revoltella RP.
Int J Dev Biol . 2007;51(8):739-43.
Select item 17825086 62.
Nadri S, Soleimani M, Kiani J, Atashi A, Izadpanah R.
Differentiation . 2008 Mar;76(3):223-31.
Select item 17653059 63.
Ono K, Yokoo S, Mimura T, Usui T, Miyata K, Araie M, Yamagami S, Amano S.
Mol Vis . 2007 Jul 13;13:1138-43.
Select item 17635514 64.
Revoltella RP, Papini S, Rosellini A, Michelini M.
Cell Prolif . 2007 Aug;40(4):445-61. Review.
Select item 17440685 65.
Safar A, Dellimore MC.
Int Ophthalmol . 2007 Oct;27(5):307-12.
Select item 17394602 66.
Baharvand H, Ebrahimi M, Javadi MA.
Dev Growth Differ . 2007 Apr;49(3):241-51.
Select item 16936093 67.
Tanioka H, Kawasaki S, Yamasaki K, Ang LP, Koizumi N, Nakamura T, Yokoi N, Komuro A, Inatomi T, Kinoshita S.
Invest Ophthalmol Vis Sci . 2006 Sep;47(9):3820-7.
Select item 16905193 68.
Nakamura T, Inatomi T, Sotozono C, Ang LP, Koizumi N, Yokoi N, Kinoshita S.
Ophthalmology . 2006 Oct;113(10):1765-72.
Select item 16779811 69.
Vascotto SG, Griffith M.
Anat Rec A Discov Mol Cell Evol Biol . 2006 Aug;288(8):921-31.
Select item 16741227 70.
Sun CC, Su Pang JH, Cheng CY, Cheng HF, Lee YS, Ku WC, Hsiao CH, Chen JK, Yang CM.
Stem Cells
Select item 16479251 71.
Talbot M, Carrier P, Giasson CJ, Deschambeault A, Guérin SL, Auger FA, Bazin R, Germain L.
Mol Vis . 2006 Feb 1;12:65-75.
Select item 16321302 72.
Lu R, Ge J, Huang B, Huang DP, Gao N, Wei YT, Wang ZC, Peng Z.
Zhonghua Yi Xue Za Zhi . 2005 Sep 21;85(36):2554-8. Chinese.
Select item 16020348 73.
Dogru M, Tsubota K.
Semin Ophthalmol . 2005 Apr-Jun;20(2):75-93. Review.
Select item 15923513 74.
Ang LP, Tan DT, Seah CJ, Beuerman RW.
Br J Ophthalmol . 2005 Jun;89(6):748-52.
Select item 15679113 75.
Pellegrini G, Dellambra E, Paterna P, Golisano O, Traverso CE, Rama P, Lacal P, De Luca M.
Eur J Cell Biol . 2004 Dec;83(11-12):691-700.
Select item 15644098 76.
Prado MR, Rocha MF, Brito EH, Girão MD, Monteiro AJ, Teixeira MF, Sidrim JJ.
Vet Ophthalmol . 2005 Jan-Feb;8(1):33-7.
Select item 15557438 77.
Homma R, Yoshikawa H, Takeno M, Kurokawa MS, Masuda C, Takada E, Tsubota K, Ueno S, Suzuki N.
Invest Ophthalmol Vis Sci . 2004 Dec;45(12):4320-6.
Select item 15371576 78.
Nishida K, Yamato M, Hayashida Y, Watanabe K, Yamamoto K, Adachi E, Nagai S, Kikuchi A, Maeda N, Watanabe H, Okano T, Tano Y.
N Engl J Med . 2004 Sep 16;351(12):1187-96.
Select item 15326108 79.
Espana EM, Di Pascuale MA, He H, Kawakita T, Raju VK, Liu CY, Tseng SC.
Invest Ophthalmol Vis Sci . 2004 Sep;45(9):2961-6.
Select item 14977781 80.
Ti SE, Grueterich M, Espana EM, Touhami A, Anderson DF, Tseng SC.
Br J Ophthalmol . 2004 Mar;88(3):422-7.
Select item 14748537 81.
Sangwan VS, Vemuganti GK, Singh S, Balasubramanian D.
Biosci Rep . 2003 Aug;23(4):169-74. Erratum in: Biosci Rep. 2006 Oct;124(10):1483.
Select item 14660463 82.
Espana EM, Ti SE, Grueterich M, Touhami A, Tseng SC.
Br J Ophthalmol . 2003 Dec;87(12):1509-14.
Select item 14660444 83.
Koizumi N, Kinoshita S.
Br J Ophthalmol . 2003 Dec;87(12):1437-9. No abstract available.
Select item 14609709 84.
Grueterich M, Espana EM, Tseng SC.
Surv Ophthalmol . 2003 Nov-Dec;48(6):631-46. Review.
Select item 12948308 85.
Ramaesh K, Dhillon B.
Eur J Ophthalmol . 2003 Jul;13(6):515-24. Review.
Select item 12827056 86.
Sangwan VS, Vemuganti GK, Iftekhar G, Bansal AK, Rao GN.
Cornea . 2003 Jul;22(5):478-81.
Select item 12564813 87.
Scuderi N, Alfano C, Paolini G, Marchese C, Scuderi G.
Scand J Plast Reconstr Surg Hand Surg . 2002;36(6):340-8.
Select item 12133553 88.
Pan Z, Zhang W, Wu Y, Sun B.
Chin Med J (Engl) . 2002 May;115(5):767-9.
Select item 12093651 89.
Shimazaki J, Aiba M, Goto E, Kato N, Shimmura S, Tsubota K.
Ophthalmology . 2002 Jul;109(7):1285-90.
Select item 12076097 90.
Meller D, Dabul V, Tseng SC.
Exp Eye Res . 2002 Apr;74(4):537-45.
Select item 10977766 91.
Basti S, Rao SK.
Curr Opin Ophthalmol . 2000 Aug;11(4):224-32. Review.
Select item 10632011 92.
Koizumi N, Inatomi T, Quantock AJ, Fullwood NJ, Dota A, Kinoshita S.
Cornea . 2000 Jan;19(1):65-71.
Select item 10460780 93.
Di Girolamo N, Tedla N, Kumar RK, McCluskey P, Lloyd A, Coroneo MT, Wakefield D.
Br J Ophthalmol . 1999 Sep;83(9):1077-82.
Select item 10330405 94.
Pellegrini G, Golisano O, Paterna P, Lambiase A, Bonini S, Rama P, De Luca M.
J Cell Biol . 1999 May 17;145(4):769-82.
Select item 10090370 95.
Cho BJ, Djalilian AR, Obritsch WF, Matteson DM, Chan CC, Holland EJ.
Cornea . 1999 Mar;18(2):216-24.
Select item 9875141 96.
Zhang S, Anderson DF, Bradding P, Coward WR, Baddeley SM, MacLeod JD, McGill JI, Church MK, Holgate ST, Roche WR.
J Pathol . 1998 Sep;186(1):59-66.
Select item 9071229 97.
Wei ZG, Lin T, Sun TT, Lavker RM.
Invest Ophthalmol Vis Sci . 1997 Mar;38(3):753-61.
Select item 8654495 98.
Chen WY, Tseng SC.
Exp Eye Res . 1995 Nov;61(5):521-34.
Select item 7531131 99.
Chen WY, Mui MM, Kao WW, Liu CY, Tseng SC.
Curr Eye Res . 1994 Oct;13(10):765-78.
Select item 8344790 100.
Lindberg K, Brown ME, Chaves HV, Kenyon KR, Rheinwald JG.
Invest Ophthalmol Vis Sci . 1993 Aug;34(9):2672-9.
Select item 8473120 101.
Wei ZG, Wu RL, Lavker RM, Sun TT.
Invest Ophthalmol Vis Sci . 1993 Apr;34(5):1814-28.
Select item 1584583 102.
Clinch TE, Goins KM, Cobo LM.
Ophthalmology . 1992 Apr;99(4):634-8.
Select item 2211036 103.
Kruse FE, Chen JJ, Tsai RJ, Tseng SC.
Invest Ophthalmol Vis Sci . 1990 Sep;31(9):1903-13.
Select item 2695344 104.
Thoft RA, Wiley LA, Sundarraj N.
Eye (Lond) . 1989;3 ( Pt 2):109-13. Review.
Select item 2424919 105.
Schermer A, Galvin S, Sun TT.
J Cell Biol . 1986 Jul;103(1):49-62.
https://www.ncbi.nlm.nih .gov/pubmed/19761393
Regen Med. 2009 Sep;4(5):677-87. doi: 10.2217/rme.09.39. Conjunctival epithelial cells maintain stem cell properties after long-term culture and …
https://www.ncbi.nlm.nih .gov/pubmed/17939121
(1)Foundation O.N.L.U.S. Stem Cells and Life, Pisa, Italy. We report on an in vitro organ culture method to investigate human conjunctival epithelial basal …
https://www.ncbi.nlm.nih .gov/pubmed/26344231
May 1, 2015 – Here we describe a simple protocol for the isolation and culture of mesenchymal stem cells (MSCs) from conjunctiva stromal tissue, with …
https://www.ncbi.nlm.nih.gov › NCBI › Literature › PubMed Central (PMC)
Apr 22, 2014 – Limbal, corneal and conjunctival stem cells are still limited for use only …. corneal lineage; the cells were transplanted after 2 weeks of culture .
https://www.ncbi.nlm.nih .gov/pubmed/25722207
Feb 26, 2015 – Human Conjunctival Stem Cells are Predominantly Located in the Medial … of stem cell markers ABCG2, ΔNp63, and Hsp70 in cultures and …
https://stemcells .nih .gov/info/basics/3.htm
Growing cells in the laboratory is known as cell culture . Human embryonic stem cells (hESCs) are generated by transferring cells from a preimplantation-stage …
https://www.ncbi.nlm.nih .gov/pubmed/10330405
Corneal stem cells are segregated in the limbus, while conjunctival stem cells … Goblet cells were found in cultures of transient amplifying cells, suggesting that …
https://www.ncbi.nlm.nih.gov › NCBI › Literature › PubMed Central (PMC)
Dec 11, 2015 – The location of the conjunctival epithelial stem cells has been investigated in several studies on mouse [15,16,17], rat [18,19], rabbit [20,21] and …
https://www.ncbi.nlm.nih.gov › NCBI › Literature › PubMed Central (PMC)
Jul 31, 2015 – Autologous limbal epithelial stem cell transplantation is the preferred treatment … Ex vivoculture of limbal, conjunctival , and oral cell biopsies.
https://www.ncbi.nlm.nih .gov/pubmed/25652765
Feb 1, 2015 – Culture medium from TNF-α-stimulated mesenchymal stem cells attenuates allergicconjunctivitis through multiple antiallergic mechanisms.
