This is a good study, though a small sample size, to show Human adipose derived stem cells may heal rat corneas faster than other options. The sample size is to small to show statistical significance. This is a positive study.
Key parts of article:
1. Owen and Friedenstein first to reported fibroblast-shaped cells in bone marrow which could differentiating into various lineages of the mesoderm in the late 1960s and named them mesenchymal stem cells (MSC).1
2. Zuck et al. showed an MSC population from Human Adipose Tissue had similar characteristics to those found in the bone marrow.2
3. Adipose-derived stem cells (ADSC) have the capability of differentiating into multiple mesodermal cell types.2,3
4. ADSC are 100 times more abundant in adipose tissue than MSC in the bone marrow.4
5. Numerous clinical trials in the past decade have reported the use of ADSC to treat acute and chronic diseases afflicting various organs.5-7
6. Arnalich-Montiel et al. first reported the successful use of ADSC-human lipoaspirate-for corneal stroma repair in a rabbit model. 8
Arnalich-Montiel F, Pastor S, Blazquez-Martinez A, Fernandez-Delgado J, Nistal M, Alio JL, et al. Adipose-derived stem cells are a source for cell therapy of the corneal stroma. Stem Cells 2008;26:570–579 [Google Scholar]7. ADSC transplanted cells were shown to be safe, non-immunogenic, preserve corneal transparency and able to differentiate into functional keratocytes.
8. References 9-10: show the inhibition of inflammation and angiogenesis in treating chemically burned rat corneas with MSC obtained from human bone marrow (Ref 9) and rat primary cell lines. (10)
Curr Eye Res.
2013 Apr;38(4):451-63. doi: 10.3109/02713683.2012.763100. Epub 2013 Feb 1.
Human adipose-derived stem cells for the treatment of chemically burned rat cornea: preliminary results.
, Salvetat ML
, Beltrami AP
, Cesselli D
, Bergamin N
, Russo R
, Cavaliere F
, Varano GP
, Alcalde I
, Merayo J
, Brusini P
, Beltrami CA
, Parodi PC
- Department of Ophthalmology, Azienda Ospedaliero Universitaria Santa Mariadella Misericordia, University of Udine, Udine, Italy. firstname.lastname@example.org
Adipose-derived stem cells (ADSC) are multipotent, safe, non-immunogenic and can differentiate into functional keratocytes in situ. The topical use of ADSC derived from human processed lipoaspirate was investigated for treating injured rat cornea.
A total of 19 rats were used. Six animals initially underwent corneal lesion experiments with 0.5 N NaOH (right eye) and 0.2 N (left). The 0.2 NaOH protocol was then used in 13 rats. All 26 eyes of 13 rats eyes received topical azythromycin bid for 3 d and divided into five treatment groups (n = 5 eyes/group), which included: control, stem cells, serum, stem + serum and adipose (raw human lipoaspirate). The four treatment groups received topical treatment three times daily for 3 d. Stem cells were isolated and harvested from human lipoaspirate. Topical eye drops were prepared daily with 1 × 10(5) cells/treatment. Fluorescein positive defect area and light microscope assessment was performed at 20, 28, 45, 50 and 74 h. Animals were sacrificed at 74 h for histological evaluation. Data were statistically analyzed for differences amongst groups.
The stem cell-treated eyes had significantly smaller epithelial defects at each time point compared to control- and adipose-treated eyes (p < 0.05). This group showed slightly better epithelium healing than the serum and combined group, yet not significantly different. Histology showed that stem cell-treated corneas had complete re-epithelization, with less inflammatory cells and limited fibroblast activation structure compared with the control eyes.
Our preliminary results show that topical treatment with ADSC seems to improve corneal wound healing.
- Owen M, Friedenstein AJ. Stromal stem cells: marrow-derived osteogenic precursors. Ciba Found Symp 1988;136:42–60
- Zuck PA, Zhu M, Ashjian P, De Ugarte DA, Huang JI, Mizuno H, et al. Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 2002;13:4279–4295
- Zuk PA. The adipose-derived stem cell: looking back and looking ahead. Mol Biol Cell 2010;21:1783–1787
- Sakaguchi Y, Sekiya I, Yagishita K, Muneta T. Comparison of human stem cells derived from various mesenchymal tissues: superiority of synovium as a cell source. Arthritis Rheum 2005;52:2521–2529
- Casteilla L, Planat-Benard V, Laharrague P, Cousin B. Adipose-derived stromal cells: Their identity and uses in clinical trials, an update. World J Stem Cells 2011;3:25–33
- Gimble JM, Guilak F, Bunnell BA. Clinical and preclinical translation of cell-based therapies using adipose tissue-derived cells. Stem Cell Res Ther 2010;1:19. doi: 10.1186/scrt19
- Philippe B, Luc S, Valerie PB, Jerome R, Alessandra BR, Louis C. Culture and use of mesenchymal stromal cells in phase i and ii clinical trials. Stem Cells Int 2010;2010:503–593
- Arnalich-Montiel F, Pastor S, Blazquez-Martinez A, Fernandez-Delgado J, Nistal M, Alio JL, et al. Adipose-derived stem cells are a source for cell therapy of the corneal stroma. Stem Cells 2008;26:570–579
- Ma Y, Xu Y, Xiao Z, Yang W, Zhang C, Song E, et al. Reconstruction of chemically burned rat corneal surface by bone marrow-derived human mesenchymal stem cells. Stem Cells 2006;24:315–321
- Oh JY, Kim MK, Shin MS, Lee HJ, Ko JH, Wee WR, et al. The anti-inflammatory and anti-angiogenic role of mesenchymal stem cells in corneal wound healing following chemical injury. Stem Cells 2008;26:1047–1055